Anti -Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1,
Gelatin Binding Protein 28, GPB28)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Purified |
E B |
|
| Acrp30, also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28kD/GBP28 or adipocyte most abundant gene transcript) was identified as a novel adipocyte-specific synthesized. It is a secreted protein with structural resemblance to complement factor C1q. Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans. Low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased Acrp30 levels. Acrp30 (mouse 247aa, rat human 244aa; chromosome 3q27) consists of a predicted NT-signal sequence 91-14aa); followed by a 27aa unique region; followed by 22 perfect Gly-X-Pro or Gly-X-X collagen like repeats; a globular segment at the C-terminus. Structurally, at the sequence level, Acrp30 resembles other collagen-like and globular domain proteins (lung surfactant protein and hepatocytes mannan-binding proteins). Acrp30 is proteolytically cleaved at 104aa to generate the globular Acrp30 (gAcrp30). Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss. A marked reduction in plasma triglycerides, glucose and free fatty acids was attributed due in part to increased fatty acid oxidation by muscle. Full length Acrp30 was less potent than gAcrp30. Therefore, gAcrp30 may open new avenues to control obesity. | | | Catalog # | A0725-17 | | Application | | | Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not ben tested. | | Recommended Dilution | ELISA (direct and indirect): 1:5000-1:10,000 | | Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at RT. | | Immunohistochemistry (Frozen): 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | 1mg/ml. | | Form | Supplied as a liquid in PBS, pH 7.4. No preservative added. | | Purity | Purified. | | Immunogen | Recombinant rat adiponectin. | | Specificity | Recognizes rat adiponectin. Cross-reactivity: mouse (weak). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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