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You are here:Home » Kits and Assays » Kits-ELISA (Obesity), BioAssay™ » Adiponectin, Human BioAssay™ ELISA Kit

Adiponectin, Human BioAssay™ ELISA Kit


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In BioAssay™ Human Adiponectin ELISA, standards, quality control and samples of sera (or plasma) are incubated in microtitration wells coated with recombinant human adiponectin together with horseradish peroxidase-labeled anti-adiponectin antibody (conjugate). After a thorough wash, the conjugate bound to the adiponectin-coated wells is allowed to react with the hydrogen peroxide/TMB substrate solution. The reaction is stopped by the addition of sulfuric acid solution and the absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is inversely proportional to the adiponectin concentration. A standard curve is constructed by plotting the absorbance values versus the concentrations of serum adiponectin standards. The concentrations of unknown samples are determined (ug/ml) using this standard curve.
Catalog #A0725-53
Kit ComponentsA0725-53A: Microtiter Strips, 1x96 wells
A0725-53B: Conjugate Solution, 1x7ml
A0725-53C:10ug/ml Standard, 1x1vial
A0725-53D: 5ug/ml Standard, 1x1vial
A0725-53E: 2ug/ml Standard, 1x1vial
A0725-53F: 1ug/ml Standard, 1x1vial
A0725-53G: 0.5ug/ml Standard, 1x1vial
A0725-53H: 0.2ug/ml Standard, 1x1vial
A0725-53J: 0.1ug/ml Standard, 1x1vial
A0725-53K: High Control, 1x400ul
A0725-53L: Low Control, 1x400ul
A0725-53M: Dilution Buffer, 2x13ml
A0725-53N: Wash Solution Concentrate (10x), 1x100ml
A0725-53P: Substrate (TMB) Solution, 2x13ml
A0725-53Q: Stop Solution, H2SO4, 1x9ml
Storage and StabilityStore all components at 4ºC. Stable for 6 months For maximum recovery of product, centrifuge the original vial and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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