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Specifications

Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including Adiponectin, involved in lipid metabolism and glucose homeostasis.
Catalog #	A0725-53S
	Adiponectin (also known as Acrp30 or Adipolean) was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Adiponectin (mouse 247aa, rat/human 244aa; chromosome 3q27) consists of a predicted NT-signal sequence 91-14aa), followed by a 27aa unique region, and then by 22 perfect Gly-X-Pro or Gly-X-X collagen like repeats, and a globular segment at the C- terminus. Structurally, adiponectin resembles other collagen-like and globular domain proteins (lung surfactant protein and hepatocytes mannan-binding proteins). Adiponectin is proteolytically cleaved at 104 aa to generate the globular Adiponectin (gAcrp30). Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss. A marked reduction in plasma triglycerides, glucose, and free fatty acids was attributed due in part to increased fatty acid oxidation by muscle. Full length adiponectin was less potent than gAcrp30. Therefore, gAcrp30 may open new avenues to control obesity.
Intended Use	Adiponectin ELISA kit is a highly sensitive sandwich type assay for the measurement of adiponectin in serum. The assay can be adapted for other biological fluids such as plasma, urine, culture medium etc.
Sensitivity	Based on 4 replicate determinations of the zero standards, the minimum Adiponectin concentration detectable using this assay is ~ 0.8ng/ml. The detection limit is defined as the value deviating by 2 SD from the zero standard.
Test Principle	Human Adiponectin ELISA kit is based on binding of Adiponectin from samples to two antibodies, one immobilized on the microtiter well plates and biotinylated detection antibody, which then binds to streptavidin-horseradish peroxidase conjugate. After a washing step, chromogenic substrate (TMB) is added and colors developed. The enzymatic reaction (blue color) is directly proportional to the amount of adiponectin present in the sample. Adding stopping solution terminates the reaction (blue color turns yellow). Absorbance is then measured on a microtiter well ELISA reader at 450 nm. and the concentration of adiponectin in samples and control is read off the standard curve.
Kit Components	Anti-Human Acrp30 coated strip plate (8 wells x 12 strips)
	Human Acrp30 Std. A (0 ng/ml), 1X0.500 ml
	Lyophilized Human Acrp30 Std. E (6ng), 2 vials (Reconstitute with 0.5 ml dH20)
	Biotin-Anti-hAcrp30 Detection Ab, (100x), 1X0.12ml (20x)
	Sample Diluent, 1X10ml
	Wash Buffer (100X), 1X10ml (100x)
	Streptavidin-HRP conjugate, 1X0.12ml
	TMB Substrate, 12 ml Stop solution, 1X12ml
Storage and Stability	Store liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.