Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin

Anti -Actin

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC IF
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% aa sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin,two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins,this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
Catalog #A0759-02A
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml
Immunohistochemistry (frozen): 4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1.2% sodium acetate . Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10K94
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a MAP backbone.
SpecificityRecognizes human Actin. Species Crossreactivity: mouse, rat, chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links