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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Sarcomeric (Skeletal, Cardiac Muscle)

Anti -Actin, alpha, Sarcomeric (Skeletal, Cardiac Muscle)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
Muscle (from Latin musculus "little mouse") is contractile tissue of the body and is derived from the mesodermal layer of embryonic germ cells. There are three types of muscles (skeletal, cardiac and smooth): Skeletal muscle or "voluntary muscle" is anchored by tendons to bone. Smooth muscle or "involuntary muscle" is found within the walls of organs and structures such as the esophagus, stomach, intestines, bronchi, uterus, urethra, bladder, and blood vessels, and unlike skeletal muscle, smooth muscle is not under conscious control. Cardiac muscle is also an "involuntary muscle" but is a specialized kind of muscle found only within the heart. Cardiac and skeletal muscle are "striated" in that they contain sarcomeres and are packed into highly-regular arrangements of bundles; smooth muscle has neither. Muscle is mainly composed of muscle cells. Within the cells are myofibrils; myofibrils contain sarcomeres, which are composed of actin and myosin. All three muscles use the movement of actin against myosin to create contraction.
Smooth muscle cells are generally arranged in sheets or bundles and connected by gap junctions. In order to contract the cells contain intracellular contractile filamentous proteins called actin and myosin. Smooth muscle does not contain the proteins troponin or titin, and caldesmon and calponin are significant proteins expressed within smooth muscle. Actin filaments attach to the sarcolemma by focal adhesions or attachment plaques and attach to other actin filaments via dense bodies (acting much like Z-lines in striated muscle).
Catalog #A0759-03A
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three alpha-actins alpha-skeletal, alpha-cardiac, and alpha-smooth muscle), one beta-actin (beta-non-muscle), and two gamma-actins (gamma-smooth muscle and gamma-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:50,000 (using 50-200ng control antigen/well)
Western Blot: 1:1500-1:2,000 (chemiluminescence)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM
Clone No11C67
HostMouse
SourceRabbit
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityAscites
ImmunogenPurified rabbit striated muscle actin protein.
SpecificityRecognizes alpha skeletal and alpha-cardiac muscle actins. Species sequence homology: rat, rabbit, human, bovine, sheep, carp, snake, frog etc. Does not react with smooth muscle tissues.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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