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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, beta

Anti -Actin, beta

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.
Catalog #A0759-05B
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 40kD band is detected in HeLa whole cell lysate and mouse NIH3T3 cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormTris-citrate/phosphate pH 7 to 8
PurityPurified by immunoaffinity chromatography.
ImmunogenThe epitope that this antibody recognizes maps to the N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709. The N-terminus of beta actin is highly conserved with gamma actin. Initial tests show that this antibody recognizes both beta and gamma actin.
SpecificityDetects a single clean band at 40kD representing actin. Species Crossreactivity: Crossreacts with Human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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