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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, Smooth Muscle (alpha-Actin 2, ACTA2)

Anti -Actin, Smooth Muscle (alpha-Actin 2, ACTA2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E B IH IC IF
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Catalog #A0759-05D
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:25-1:50 (cells) and 1:100 (vessel sections)
Immunohistochemistry (FFPE or frozen): 1:50-1:75. Incubate for 30 minutes at RT.
Immunocytochemistry: 1:75
Optimal dilutions to be determined by the researcher.
Positive ControlLeiomyoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No8J164
HostMouse
ConcentrationNot Determined
FormSupplied as a liquid in 20mM Tris-borate, 150mM sodium chloride, 0.09% sodium azide.
PuritySupernatant
ImmunogenN-terminal decapeptide of alpha-smooth muscle actin. Cellular Localization: Cytoplasmic
SpecificityRecognizes the alpha smooth muscle isoform of human actin, smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, and ovary. Does not react with actin from fribroblasts, striated muscle or myocardium.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesACTSA, ACTVS, ACTSA, alpha Smooth Muscle Actin


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