| Actin is an abundant cytoskeletal protein found in all cells. The protein’s 42kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction. In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis and phagocytosis. Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific. |
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| Catalog # | A0759-07 |
| Applications | Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 1:5000; HEK293 cell lysates were probed with A0759-07 and visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. |
| Immunohistochemistry: 10ug/ml (paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections). |
| Immunocytochemistry (IF): 1:500; HeLa and NIH/3T3 cells were probed with A0759-07 and DAPI for nuclear staining. Visualized using an gG (Cy3) donkey anti-mouse. Positive staining pattern reflects both membrane and cytoplasmic staining. |
| ELISA: Shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | N/A |
| Clone Type | Monoclonal |
| Isotype | IgG2b,k |
| Clone No | 2Q1055 |
| Host | Mouse |
| Source | Chicken |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Purified chicken gizzard actin. Epitope: Appears to be located in the N-terminal two thirds of the actin molecule, possibly near aa50-70. |
| Specificity | Recognizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms. This clone is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. Labels myotubes and stains myoblasts and fibroblasts. Does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. Species Crossreactivity: All vertebrates as well as Dictyostelium discoideum and Physarum polycephalum. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
