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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B IH IF Gel

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex. It has been reported that actin is hyperphosphorylated in primary breast tumors. It has also been shown that actin dysfunction leads to certain types of heart failure.
Catalog #	A0759-08M
Applications	Suitable for use in Indirect Immunofluorescence, ELISA, Western Blot Dot Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Indirect Immunofluorescence: 1:200-1:2000. Staining of cultured human or chicken fibroblasts
	Western Blot: 1:500 using Human or chicken fibroblast extract. Band at ~ 42kD.
	Immunohistochemistry (Paraffin): Methanol-fixed sections. Zinc-formalin, B5, ethanol, methacarn, Brunnel's or Bouin's solution can be used as fixatives. Can also be used on frozen sections.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	8J165
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid, 15mM sodium azide
Purity	Ascites
Immunogen	Synthetic peptide, SGPSIVHALCP corresponding to human actin attached to Multiple Antigen Peptide backbone. Epitope
Specificity	Recognizes human actin. Species Crossreactivity: mouse, rat, bovine, chicken, canine, goat, guinea pig, porcine, rabbit, amphibian and sheep Does not react with the epitope on the N-terminal end of actin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	ActG, ActG1, DFNA20