Anti -Actin, pan (alpha, beta, gamma) (FITC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IF |
|
| Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are ~90% conserved, except in the N-terminal 18aa (50-60%homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots. | | | Catalog # | A0759-10D | | Applications | Suitable for use in Immunofluorescence. Other applications not tested. | | Recommended Dilutions | Immunofluorescence: 1:50-1:200 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 11C68 | | Host | Mouse | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. Labeled with Fluorescein isothiocyanate (FITC) | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to 11aa SGPSIVHRKCF near the C terminal of human actin. | | Specificity | Recognizes human Actin. Species Crossreactivity: bovine, sheep, goat, porcine, rabbit, canine, mouse, rat, guinea pig, hamster, chicken, carp, frog and snail. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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