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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, pan (alpha, beta, gamma) (Biotin)

Anti -Actin, pan (alpha, beta, gamma) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are ~90% conserved, except in the N-terminal 18aa (50-60%homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.
Catalog #A0759-10E
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:5,000-1:30,000
Western Blot: 1:2,000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No11C68
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA., 0.05% sodium azide, Labeled with Biotin using Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). F/P ratio
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to human 11-aa-MAPS (SGPSIVHRKCF) of actin.
SpecificityRecognizes human Anti-Actin
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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