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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, pan (ACT1, 2, 3, 4, 7, 8, 11, 12)

Anti -Actin, pan (ACT1, 2, 3, 4, 7, 8, 11, 12)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. In plants, exogenous application of hormones initiates a variety of processes directed by the cytoskeleton, such as the initiation of rapid cell proliferation, cell expansion, and differentiation. Higher plants contain actins encoded by a relatively ancient and highly divergent multigene family. Arabidopsis has eight functional actin genes. On the basis of their sequence and expression, they have been grouped into two major phylogenetic classes, reproductive and vegetative, and to five subclasses (two for vegetative and three for reproductive). The reproductive actin genes ACT1, 3, and 11 are expressed strongly in mature pollen, growing pollen tubes, ovules, and to a certain extent in embryos, and young meristematic tissue. The vegetative actin (ACT2, 4, 7, 8, and 12) differ from the reproductive actins by 4- 7% at the aa level and show distinct expression patterns. The pairs ACT1 and ACT3, and ACT2 and ACT8, differ only in 1aa. Misexpression of ACT1 but not ACT2 induced dwarfism in plants in addition to delayed flowering, significantly reduced organ size and altered branching pattern of the leaf trichomes and inflorescence stem. During cell differentiation and maturation in plants, there is a developmental switch in the regulation of actin isovariants. For example, during Arabidopsis and tobacco pollen development, there is a switch from completely vegetative to predominantly reproductive actin isovariants. Furthermore, different cell types may differ in their preference for actin isovariants to fulfill their distinct cellular functions. In Arabidopsis ACT7 isovariant is essential for normal phytohormone response to hormones in different organs.
Catalog #A0759-10G
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-50,000; using 50-200ng control antigen/well
Western Blot: 1:1500-1:2,000 (chemiluminescence)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No11C72
HostMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityAscites
ImmunogenPurified plant actin protein (ACT8).
SpecificityRecognizes actin in many plant species and in Dictyostelium (~43-45kD). In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11, and 12).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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