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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin

Anti -Actin

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B IC
The Actin protein, a component of the eukaryotic cytoskeleton, is highly conserved between multiple species. Levels of the Actin protein may vary between different cell types, but are consistent within a given cell type, and for this reason, Actin protein levels are considered an internal control for protein loading.
Applications:
Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested.
Catalog #A0759-11G
Recommended DilutionsImmunocytochemistry: 5-15ug/ml. Stains immersion fixed HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line.
Western Blot: 1ug/ml. HeLa human cervical epithelial carcinoma cell line, MCF­7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC­12 rat adrenal pheochromocytoma cell line
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman platelet derived Actin
SpecificityRecognizes human Actin. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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