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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, F (Filamentous)

Anti -Actin, F (Filamentous)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH FC
Catalog #A0759-14
ApplicationsSuitable for Flow Cytometry, Immunohistochemistry, Western Blot and ELISA. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:5
Immunohistochemistry (Frozen): 1:50-1:250
ELISA: 1:5
Optimal dilutions to be determined by the researcher.
Positive ControlGastrointestinal tissue
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No4i374
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified immunoglobulin from tissue culture supernatant
ImmunogenHuman monocytes and U397 cell line
SpecificityRecognizes human Filamentous actin (F-actin). Binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. Recognizes a 43kD band in Western Blot. Species Crossreactivity: Rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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