| Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbilical cord. |
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| Catalog # | A0759-25 |
| Applications | Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:500 (see Note below). |
| Western Blot: 1:1000 (using the ECL-enhanced procedure) |
| Note: It is necessary to include 1M sodium chloride for application in Western Blot and ELISA to enhance specificity. |
| Immunohistochemistry (frozen and paraffin): 1:10. It is necessary to include 0.5M sodium chloride in all washing buffers for application on frozen tissue. This is not necessary for paraffin sections. Microwave treatment is necessary for paraffin sections. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5F152 |
| Host | Mouse |
| Concentration | ~0.05mg/ml (after reconsitution) |
| Form | Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic N-terminus decapeptide of cardiac isoform of actin. |
| Specificity | Recognizes fetal cardiac actin. Represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) a-actin from all other actin isoforms. Shows no crossreaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied. |
| Species Crossreactivity | Human, bovine, rabbit, chicken |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
