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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Actin, Cardiac, Fetal

Anti -Actin, Cardiac, Fetal


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbilical cord.
Catalog #A0759-25
ApplicationsSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:500 (see Note below).
Western Blot: 1:1000 (using ECL)
Note: It is necessary to include 1M sodium chloride for application in Western Blot and ELISA to enhance specificity.
Immunohistochemistry (Frozen, Paraffin): 1:10. It is necessary to include 0.5M sodium chloride in all washing buffers for application on frozen tissue. This is not necessary for paraffin sections. Microwave treatment is necessary for paraffin sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS/ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
Clone No5F152
Concentration~0.05mg/ml (after reconsitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic N-terminus decapeptide of cardiac isoform of actin.
SpecificityRecognizes fetal cardiac actin. Represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) a-actin from all other actin isoforms. Shows no crossreaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied.
Species CrossreactivityHuman, bovine, rabbit, chicken
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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