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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin (ACTA2, ACTSA, actin, alpha 2, smooth muscle, aorta, alpha-cardiac actin)

Anti -Actin (ACTA2, ACTSA, actin, alpha 2, smooth muscle, aorta, alpha-cardiac actin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IF
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Catalog #A0760-04B
ApplicationsSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionPeptide ELISA: 1:4000
Western Blot: 0.1-0.3ug/ml. A band of ~45kD observed in human Duodenum lysates.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to human Actin.
SpecificityRecognizes human Actin. Species Crossreactivity: Canine, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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