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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Actin F (Filamentous)

Anti -Actin F (Filamentous)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH FC
Catalog #A0760-21
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:10
ELISA: 1:10
Western Blot: 1:100-1:500
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
HybridomaNS1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM
Clone No8.S2.1
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide
PurityPurified
ImmunogenHuman monocytes and U937 cell line.
SpecificityRecognizes human Filamentous Actin. Binds to the N-terminal region of actin, but not to the extreme N-terminal 40aa. In tissue sections, stains the cytoplasm of macrophages strongly and gives granular, localized nuclear staining of all cell types.
Species CrossreactivityRabbit, rat and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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