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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IH FC

Catalog #	A0760-21
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution	Flow Cytometry: 1:5
	Immunohistochemistry: frozen sections
	ELISA: 1:5
	Optimal dilutions to be determined by the researcher.
Hybridoma	Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Recommended Positive Control	Gastrointestinal tissue
Recommended Secondary Ab	I1905-28A: Goat anti-mouse IgM (HRP)
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	8.S2.1
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, before the addition of glycerol to 40%.
Purity	Purified IgM prepared from tissue culture supernatant.
Immunogen	Human monocytes and U937 cell line.
Specificity	Recognizes human Filamentous Actin. Binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly and gives granular, localized nuclear staining of all cell types. Species Crossreactivity: Rabbit.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.