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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant IH

Actin is a major component of the cytoskeleton. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. This antibody is useful in the identification of rhabdoid cellular elements.
Catalog #	A0760-22
Applications	Suitable for use in Immunohistochemistry.Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
	Optimal dilution to be determined by the researcher.
Positive Control	Muscle
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	0.N.3
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid, 0.09% sodium azide.
Purity	Supernatant
Immunogen	SDS extract of human myocardium.
Cellular Localization	Cytoplasmic
Specificity	Recognizes human actin from tissue extracts of uterus, ileum, aorta, diaphragm, heart, and smooth muscle cells. Recognizes alpha actin from skeletal, cardiac, and smooth muscle, and gamma actin from smooth muscle sources. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Species Crossreactivity	Rabbit and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.