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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, Muscle Specific

Anti -Actin, Muscle Specific

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
Actin is a major component of the cytoskeleton. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. This antibody is useful in the identification of rhabdoid cellular elements.
Catalog #A0760-22
ApplicationsSuitable for use in Immunohistochemistry.Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilution to be determined by the researcher.
Positive ControlMuscle
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.3
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.09% sodium azide.
PuritySupernatant
ImmunogenSDS extract of human myocardium.
Cellular LocalizationCytoplasmic
SpecificityRecognizes human actin from tissue extracts of uterus, ileum, aorta, diaphragm, heart, and smooth muscle cells. Recognizes alpha actin from skeletal, cardiac, and smooth muscle, and gamma actin from smooth muscle sources. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Species CrossreactivityRabbit and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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