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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, Muscle Speci c (BSA & Azide Free)

Anti -Actin, Muscle Speci c (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
Catalog #A0760-22AX
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Formalin and paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues.
Optimal dilutions to be determined by the researcher.
Positive ControlMuscle or sarcoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F10
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See A0760-22A.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified actin
SpecificityRecognizes alpha-smooth muscle as well as alpha-skeletal and alpha-cardiac (sarcomeric) isoforms of human actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas). Species Crossreactivity: mouse, rat, porcine, equine, bovine, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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