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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, Muscle Specific (Muscle Actin, ACTA, ACTA1, ACTC, ACTSA, Alpha 2 Actin, ACTA2, Cardiac Muscle alpha Actin 1, ACTC1, Skeletal Muscle alpha Actin 1)

Anti -Actin, Muscle Specific (Muscle Actin, ACTA, ACTA1, ACTC, ACTSA, Alpha 2 Actin,
ACTA2, Cardiac Muscle alpha Actin 1, ACTC1, Skeletal Muscle alpha Actin 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E IH
Actin is a major component of the cytoskeleton. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. This antibody is useful in the identification of rhabdoid cellular elements.
Catalog #A0760-22D
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Paraffin, frozen): 1:100-1:500
Optimal dilutions to be determined by the researcher.
Positive ControlSkeletal Muscle
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone NoHHF35
HostMouse
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in PBS, pH7.4, protein base, sodium azide.
PurityAscites
ImmunogenHuman Actin, Muscle Specific.
SpecificityRecognizes human Actin, Muscle Specific of skeletal, cardiac, and smooth muscle cells. Not reactive with other mesenchymal cells except for myoepithelium. Staining pattern: Cytoplasmic.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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