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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH

Actin is a semi-flexible polymer found as a part of the cytoskeleton of cells in higher biological organisms. It is a highly conserved protein which participates in a wide variety of cellular functions in eucaryotes including muscle contraction, amoeboid movement, cytokinesis, and mitotic division. Actin protein as a polymer without attached proteins is called filamentous actin (F-actin), while the globular actin monomers are called G-actin. Humans have six actin genes; two striated muscle types or sarcomeric (alpha-skeletal and alpha-cardiac), two smooth muscle types ands two cytoskeletal forms (beta and gamma-types). Alpha-actin is found in muscle cells where it plays an important role in contracting the cell. Whereas beta-actin is localized in the front of moving cells and gamma-actin forms stress fibers. The cytoplasmic beta and gamma actins are coexpressed in mammalian non-muscle cells, while the two smooth muscle actins are coexpressed only in smooth muscle with their relative amounts varying in different smooth muscle types. Similarly the alpha-skeletal actin is thought to be the only actin expressed in adult skeletal muscle, and alpha-cardiac actin is thought to be the only actin expressed in adult heart muscle.
Catalog #	A0760-22E
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:75
	Immunohistochemistry: Frozen
	Optimal dilutions to be determined by the researcher.
Positive Control	Skeletal muscle
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	11E5
Host	Mouse
Source	Human
Concentration	Not determined.
Form	Supplied as a liquid, 0.05% sodium azide.
Purity	Supernatant
Immunogen	SDS extracted protein from human myocardium. Cellular Localization: Cytoplasmic.
Specificity	Recognizes human Actin, muscle.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.