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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH IF

Actin is a highly conserved skeletal protein which is in both muscle and nonmuscle cells. The mammalian tissues express at least 6 unique actin isotypes. Alpha isotype is found exclusively in muscle cells including alpha-skeletal, alpha-cardiac, and alpha and gamma smooth muscle actin. Beta and gamma nonmuscular actins have been observed in non-muscle cells.
Catalog #	A0760-26
Applications	Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry (Formalin/paraffin): 1:800 for 20 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
	Optimal dilutions to be determined by the researcher.
Positive Control	Smooth muscle or leiomyosarcoma
Source	Ascites
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	0.N.5
Host	Mouse
Source	Human
Concentration	~0.04mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See A0760-26X.
Purity	Purified by Protein A affinity chromatography.
Immunogen	N-terminal decapeptide of the alpha smooth muscle isoform of Actin; acetylated at the N-terminus. Epitope
Specificity	Recognizes human alpha-smooth muscle isoform of actin. Stains smooth muscle cells in vessel walls, gut wall and myometrium. Myoepithelial cells in breast and salivary gland are also stained. Reacts with tumors arising from smooth muscles and myoepithelial cells. Species Crossreactivity: baboon, bovine, rabbit, mouse, rat and chicken
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.