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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Smooth Muscle

Anti -Actin, alpha, Smooth Muscle

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
Actin is a highly conserved skeletal protein which is in both muscle and nonmuscle cells. The mammalian tissues express at least 6 unique actin isotypes. Alpha isotype is found exclusively in muscle cells including alpha-skeletal, alpha-cardiac, and alpha and gamma smooth muscle actin. Beta and gamma nonmuscular actins have been observed in non-muscle cells.
Catalog #A0760-26
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Formalin/paraffin): 1:800 for 20 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
Optimal dilutions to be determined by the researcher.
Positive ControlSmooth muscle or leiomyosarcoma
SourceAscites
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No0.N.5
HostMouse
SourceHuman
Concentration~0.04mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See A0760-26X.
PurityPurified by Protein A affinity chromatography.
ImmunogenN-terminal decapeptide of the alpha smooth muscle isoform of Actin; acetylated at the N-terminus. Epitope
SpecificityRecognizes human alpha-smooth muscle isoform of actin. Stains smooth muscle cells in vessel walls, gut wall and myometrium. Myoepithelial cells in breast and salivary gland are also stained. Reacts with tumors arising from smooth muscles and myoepithelial cells. Species Crossreactivity: baboon, bovine, rabbit, mouse, rat and chicken
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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