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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Smooth Muscle (Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell Growth-inhibiting Gene 46 Protein, ACTA2, ACTSA, ACTVS, GIG46)

Anti -Actin, alpha, Smooth Muscle (Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell
Growth-inhibiting Gene 46 Protein, ACTA2, ACTSA, ACTVS, GIG46)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24. Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.
Catalog #A0760-26B
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 2ug/ml
Immunohistochemistry (Formalin or acetone fixed): 0.3-1.2ug/ml
Immunohistochemistry: Formalin fixed paraffin embedded
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1.2% sodium acetate or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10K95
HostMouse
SourceHuman
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1ml 1.2% sodium acetate or PBS.
PurityPurified by affinity chromatography.
ImmunogenN-terminal synthetic decapeptide of alpha-smooth muscle actin.
SpecificityRecognizes human Smooth Muscle Actin, alpha. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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