Anti -Actin, alpha, Smooth Muscle (Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell
Growth-inhibiting Gene 46 Protein, ACTA2, ACTSA, ACTVS, GIG46)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH IF |
|
| Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24. Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins. | | | Catalog # | A0760-26B | | Applications | Suitable for use in Western Blot, Immunohistochemistry (frozen, paraffin), Immunoprecipitation, and Immunofluorescence. Other applications not tested. | | Recommended Dilution | Western Blot: 2ug/ml | | Immunohistochemistry (frozen): 0.3-1.2ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1.2% sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 10K95 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | N-terminal synthetic decapeptide of alpha-smooth muscle actin. | | Specificity | Recognizes human Smooth Muscle Actin, alpha. Species Crossreactivity: mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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