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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -ACTA2 (Actin, alpha Smooth Muscle, Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell Growth-inhibiting Gene 46 Protein, ACTSA, ACTVS, GIG46)

Anti -ACTA2 (Actin, alpha Smooth Muscle, Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell
Growth-inhibiting Gene 46 Protein, ACTSA, ACTVS, GIG46)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
Actin is a highly conserved protein which participates in a wide variety of cellular functions in eucaryotes including muscle contraction, amoeboid movement, cytokinesis, and mitotic division. Humans have six actin isotypes; two striated muscle types or sarcomeric (alpha-skeletal and alpha-cardiac), two smooth muscle types and two cytoskeletal forms (beta and gamma-types). Each isotypes is coded by a separate gene and is expressed in a developmentally regulated and tissue-specific manner. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. The smooth muscle alpha-actin is expressed in vascular smooth muscle cells and fibroblast cells. Its expression is regulated by hormones, cell proliferation and repressed by pathological conditions like oncogenic transformation and artherosclerosis. It maps to 10q23.3 region of human chromosome. Northern blot analysis revealed that human smooth muscle cells express only a single 1.4 kilobase smooth muscle alpha actin transcript.
Catalog #A0760-26J
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.3-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Duodenum lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to EEEDSTALVC, from the N-terminus of the protein sequence according to NP_001604.1.
SpecificityRecognizes human Smooth Muscle Actin. Species sequence homology: mouse, rat, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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