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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Smooth Muscle (BSA, Glycerol & Azide Free)

Anti -Actin, alpha, Smooth Muscle (BSA, Glycerol & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.
Catalog #A0760-26X
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Frozen & formalin/paraffin): 0.5-1ug/ml for 30min at RT. No special pretreatment is required for the Immunohistochemical staining of formalin/paraffin tissues.
Optimal dilutions to be determined by the researcher.
Positive ControlSmooth muscle or leiomyosarcoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No0.N.5
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservatives added. No stabilizing proteins added. Glycerol free. Also available with BSA and azide. See cat# A0760-26.
PurityPurified by Protein A affinity chromatography.
ImmunogenN-terminal decapeptide of the alpha smooth muscle isoform of actin; acetylated at the N-terminus. Epitope Location
SpecificityRecognizes the alpha-smooth muscle isoform of actin. Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. Reacts with tumors arising from smooth muscles and myoepithelial cells. Does not crossreact with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial). Species Crossreactivity: Human, baboon, bovine, rabbit, mouse, rat and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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