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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Smooth Muscle

Anti -Actin, alpha, Smooth Muscle

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation, cultured stress fibers of smooth muscle-derived cells, and some smooth muscle subtype fibroblasts.
Catalog #A0760-27B
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (frozen and paraffin-embedded tissue as well as cytological material). Other applications not tested.
Recommended DilutionsImmunohistochemistry: Ready-to-use on frozen sections. Paraffin embedded sections require protease pretreatment prior to antibody application. Incubate 1 hour at RT; extended with paraffin.
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5F160
HostMouse
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic N-terminus decapeptide of alpha-smooth-muscle isoform of actin.
SpecificityRecognizes alpha-smooth-muscle isoform of actin (Mr 43,000). Reactivities on cultured stress fibers of smooth muscle-derived cells. Tumors specificallly detected
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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