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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Smooth muscle

Anti -Actin, alpha, Smooth muscle

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites IH
Actin is a highly conserved skeletal protein which is in both muscle and nonmuscle cells. The mammalian tissues express at least 6 unique actin isotypes. Alpha isotype is found exclusively in muscle cells including alpha-skeletal, alpha-cardiac, and alpha and gamma smooth muscle actin. Beta and gamma nonmuscular actins have been observed in non-muscle cells.
Catalog #A0760-27C
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: Formalin-fixed paraffin-embedded human tissue sections, cryostat sections(1), or cell smears
Optimal dilutions to be determined by the researcher.
Positive Control Leiomyosarcoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A3
HostMouse
Concentration~0.05mg/ml
FormSupplied as a liquid in PBS, pH 7.4, and 1% BSA with 0.1% sodium azide before the addition of glycerol to 40%.
PurityMouse ascites
ImmunogenActin synthetic peptide. Expected Staining Pattern: Cytoplasmic
SpecificityRecognizes alpha isoform of smooth muscle actin. Does not cross react with skeletal or cardiac muscle actin, mesenchymal cells, epithelial cells, or other cytoskeletal components. Reacts with smooth muscle actin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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