Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites IH

Actin is a highly conserved skeletal protein which is in both muscle and nonmuscle cells. The mammalian tissues express at least 6 unique actin isotypes. Alpha isotype is found exclusively in muscle cells including alpha-skeletal, alpha-cardiac, and alpha and gamma smooth muscle actin. Beta and gamma nonmuscular actins have been observed in non-muscle cells.
Catalog #	A0760-27C
Applications	Suitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry: Formalin-fixed paraffin-embedded human tissue sections, cryostat sections(1), or cell smears
	Optimal dilutions to be determined by the researcher.
Positive Control	Leiomyosarcoma
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	6A3
Host	Mouse
Concentration	~0.05mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, and 1% BSA with 0.1% sodium azide before the addition of glycerol to 40%.
Purity	Mouse ascites
Immunogen	Actin synthetic peptide. Expected Staining Pattern: Cytoplasmic
Specificity	Recognizes alpha isoform of smooth muscle actin. Does not cross react with skeletal or cardiac muscle actin, mesenchymal cells, epithelial cells, or other cytoskeletal components. Reacts with smooth muscle actin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.