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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Smooth Muscle

Anti -Actin, alpha, Smooth Muscle

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IC FC
Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.
Catalog #A0760-27D
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 2.5ug/10e6 cells using human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin
Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human heart and human breast cancer tissue.
Immunocytochemistry: see references 5 and 6
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9H14
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein A affinity chromatography.
ImmunogenN-terminal synthetic decapeptide of alpha-Smooth muscle actin
SpecificityRecognizes human alpha-Smooth Muscle Actin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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