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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Alpha actins are found mainly in muscle tissues.
Catalog #	A0760-27E
Applications	Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 2ug/ml Human smooth muscle actin has a predicted length of 377 residues and a MW of 42kD.
	Immunohistochemistry (formalin fixed and paraffin): 0.2-2ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, pH 7.4, to concentration of 0.1-1mg/ml.. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9G306
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.02% sodium azide. Reconstitute in 1ml of PBS to concentration of 0.1-1mg/ml.
Purity	Purified by affinity chromatography.
Immunogen	A synthetic peptide of 10 amino acids corresponding to the N-terminus of alpha smooth muscle actin.
Specificity	Reacts only with alpha smooth muscle actin and does not react with actin from striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It also does not react with beta and gamma actins. Species crossreactivity: Human, mouse, porcine, baboon, rabbit.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	ACTA2, ACTSA, ACTVS