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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Smooth Muscle

Anti -Actin, alpha, Smooth Muscle

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Alpha actins are found mainly in muscle tissues.
Catalog #A0760-27E
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml Human smooth muscle actin has a predicted length of 377 residues and a MW of 42kD.
Immunohistochemistry (formalin fixed and paraffin): 0.2-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, pH 7.4, to concentration of 0.1-1mg/ml.. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9G306
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.02% sodium azide. Reconstitute in 1ml of PBS to concentration of 0.1-1mg/ml.
PurityPurified by affinity chromatography.
ImmunogenA synthetic peptide of 10 amino acids corresponding to the N-terminus of alpha smooth muscle actin.
SpecificityReacts only with alpha smooth muscle actin and does not react with actin from striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It also does not react with beta and gamma actins. Species crossreactivity: Human, mouse, porcine, baboon, rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesACTA2, ACTSA, ACTVS


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