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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Smooth Muscle

Anti -Actin, alpha, Smooth Muscle

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.
Catalog #A0760-30
ApplicationsSuitable for use in Immunohistochemistry, Immunofluorescence, and Western Blot. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Frozen and Formalin fixed paraffin embedded sections): 1:2000. Protease treatment is required for paraffin fixed samples. Stains leiomyosarcomas, leiomyomas, certain stromal cells surrounding infiltrating ductal carcinoma of the breast.
Optimal dilution to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q1017
HostMouse
SourceHuman
Concentration~0.05mg/ml (after reconstitution)
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin.
SpecificitySpecific for the alpha-smooth muscle isoform of actin (Mr 43kD). Recognizes many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Species Crossreactivity: human, mouse and rat, bovine, equine and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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