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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Smooth Muscle

Anti -Actin, alpha, Smooth Muscle

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
Alpha Smooth muscle actin is found in smooth muscle cells such as those in blood vessel wall regions of the intestine and myoepithelial glands. This antibody may be useful as a myogenic marker or to identify smooth muscle differentiation.
Catalog #A0760-30A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:100-1:250
Immunohistochemistry: 1:25-1:50 for 1 hr at RT
Optimal dilutions to be determined by the researcher.
Positive Control
Western Blot: Smooth muscle.
Immunohistochemistry: Bowel wall, with distinct labeling of the muscularis mucosae and the muscularis propria (cytoplasmic staining pattern)
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone NoAsm-1
HostMouse
ConcentrationNot determined
FormSupplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
PuritySupernatant
ImmunogenSynthetic peptide of 10aa representing the amino terminal of the alpha smooth muscle isoform of actin.
SpecificityRecognizes Smooth Muscle Actin, Alpha. Species Crossreactivity: Human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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