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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Sarcomeric

Anti -Actin, alpha, Sarcomeric

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IH
Catalog #A0760-30F
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: (frozen)- Neat, (paraffin)- Neat. Does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.Staining of formalin fixed paraffin embedded tissues may be improved in some cases by the use of antigen retrieval techniques.
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsF(ab')2 rabbit anti-mouse IgG HRP
Recommended Positive Control TissueHeart, Striated muscle
Recommended Negative ControlsMouse IgM Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No3H516
HostMouse
SourceHuman
ConcentrationReady to use
FormSupplied as a liquid, 1% BSA, 0.09% sodium azide.
PurityPurified
ImmunogenPurified rabbit skeletal muscle Actin.
SpecificityRecognizes alpha-skeletal and alpha-cardiac actins (the sarcomeric actins). No reactivity with smooth muscle actin is seen. Species Crossreactivity: Broad.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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