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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E IH

Actin is a major component of the cytoskeleton and is present in every cell type. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Smooth Muscle Actin Ab does not stain cardiac or skeletal muscle, however, it will stain myofibroblasts and myoepithelial cells. This antibody could be used together with Muscle Specific Actin to distinguish leiomyosarcoma from rhabdomyosarcoma. In most cases of rhabdomyosarcoma, this antibody gives negative results whereas M. S. Actin is positive in the rhabdomyoblasts. Leiomyosarcomas are positive with both M. S. Actin and S. M. Actin antibodies.
Catalog #	A0760-31A
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (Paraffin, frozen): 1:10-1:50 Required Heat Induced Epitope Retrieval (HIER) for paraffin.
	Optimal dilutions to be determined by the researcher.
Positive Control	Appendix, Uterus, Vessel Wall
Staining Pattern	Cytoplasmic
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG,k
Clone No	1A4
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, <0.1% sodium azide.
Purity	Ascites
Specificity	Recognizes human Actin, Smooth Muscle.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.