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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IF

Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At
least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern Blots.
Catalog #	A0760-39B
Applications	Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilution	ELISA: 1:10,000-1:50,000
	Western Blot: 1:1500-1:2000
	Immunofluorescence: 1:500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9j104
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Purity	Purified.
Immunogen	Synthetic peptide corresponding to 15aa near the N-terminal of human beta-actin (non-muscle) (KLH).
Specificity	Recognizes human Beta-Actin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.