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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Beta actins coexist with gamma actins in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Catalog #	A0760-39D
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 0.5-1ug/ml
	Immunohistochemistry (formalin, paraffin, and acetone): 0.2-0.5ug/ml
	Optimal dilution determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9G307
Host	Mouse
Concentration	As reported
Form	Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
Purity	Purified by affinity chromatography.
Immunogen	A modified synthetic peptide (C-DDIALVIDNGSGK) corresponding to the N-terminus of beta Actin conjugated to KLH.
Specificity	Recognizes human, mouse, and rat ACTB. Does not react with alpha and gamma actins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	ACTB, AX, actin, Actx,