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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Actin, beta

Anti -Actin, beta


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Clone Host Grade Applications
Monoclonal Mouse Purified E B IH IF
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western Blot. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Catalog #A0760-40
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.5-1ug/ml. Useful as a loading control for a wide variety of cell extracts including human foreskin fibroblasts or chicken fibroblasts.
Immunohistochemistry (Paraffin and frozen): The epitope recognized by the antibody is resistant to paraffin or formalin-fixed tissue sections. Acetone fixation or B5, Methacarn, ethanol or Bouinís solution may also be used as fixatives.
Immunofluorescence (IC): 10-40ug/ml on methanol/acetone fixed samples. Staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membranes associated dense-plaque.
Optimal dilutions to be determined by the researcher.
Positive Control
Cultured human or chicken fibroblasts
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No2A2.1
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
ImmunogenSynthetic peptide corresponding to Ac-Asp-Asp-Asp-Ile-Ala-Ala- Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys from beta Actin at N-terminal, conjugated to KLH. Molecular Weight: 42kD.
SpecificityRecognizes beta Actin in a wide variety of tissues and species. Does not crossreact with adult cardiac or skeletal muscle or amoeba beta actin. Species Crossreactivity: human, bovine, sheep, porcine, rabbit, feline, canine, mouse, rat, guinea pig, chicken, fish (carp) and leech.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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