| Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western Blot. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. |
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| Catalog # | A0760-40 |
| Applications | Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 0.5-1ug/ml. Useful as a loading control for a wide variety of cell extracts including human foreskin fibroblasts or chicken fibroblasts. |
| Immunohistochemistry (Paraffin and frozen): The epitope recognized by the antibody is resistant to paraffin or formalin-fixed tissue sections. Acetone fixation or B5, Methacarn, ethanol or Bouin’s solution may also be used as fixatives. |
| Immunofluorescence (IC): 10-40ug/ml on methanol/acetone fixed samples. Staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membranes associated dense-plaque. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | |
| Cultured human or chicken fibroblasts |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2A2.1 |
| Host | Mouse |
| Source | Xenopus |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. |
| Purity | Purified |
| Immunogen | Synthetic peptide corresponding to aa1-14, DDDIAALVIDNGSGL of Xenopus laevis beta Actin, conjugated to KLH. Epitope |
| Specificity | Recognizes Xenopus beta Actin in a wide variety of tissues and species. Does not crossreact with adult cardiac or skeletal muscle or amoeba beta actin. Species Crossreactivity: human, bovine, sheep, porcine, rabbit, feline, canine, mouse, rat, guinea pig, chicken, fish (carp) and leech. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
