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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, beta

Anti -Actin, beta

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC IF
Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Catalog #A0760-40A
ApplicationsSuitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 1:100-1:2000. Can be blocked with beta Actin peptide (aa2-15). At higher concentrations, other bands can be detected. Note: May be used as a cell extract loading control.
Immunofluorescence: 1:50.
Immunohistochemistry: 1:100
Immunohistochemistry (Paraffin): 1:100 Requires HIER with sodium citrate buffer pH6.0
Immunoprecipitation: 1:10-1:500
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa whole cell lysate and 3T3 cells (mouse). These were negative when used in Immunocytochemistry.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Note: We do not recommended adding glycerol as a cryopreservative.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-citrate, phosphate buffer, pH 7-8, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the N-terminus of human beta Actin. Cellular Localization: Cytoplasmic.
SpecificityRecognizes human beta Actin. Species Crossreactivity: mouse, rat, monkey. Expected to crossreact with a wide range of species due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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