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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP IC IF

Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Catalog #	A0760-40A
Applications	Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended Dilution	Western Blot: 1:500-1:2500. Can be blocked with beta Actin peptide (aa2-15). At higher concentrations, other bands can be detected. Note: May be used as a cell extract loading control.
	Immunofluorescence: 1:50.
	Optimal dilutions to be determined by the researcher.
Positive Control	HeLa whole cell lysate and 3T3 cells (mouse).
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in Tris-citrate, phosphate buffer, pH 7.0, 0.09% sodium azide, before the addition of glycerol to 40%.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to the N-terminus of human beta Actin. Cellular Localization: Cytoplasmic.
Specificity	Recognizes human beta Actin. Species Crossreactivity: mouse, rat, monkey. Expected to crossreact with a wide range of species due to sequence homology.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.