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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)

Anti -Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IF
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Catalog #A0760-40H1
ApplicationsSuitable for use in Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 1:1,000-4,000
Immunofluorescence: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10K96
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified.
ImmunogenBeta-cytoplasmic actin N-terminal peptide.
SpecificityRecognizes human Beta-Actin. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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