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Abs to Actin Related Proteins
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Anti -Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)
Anti -Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH |
|
| Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. | | | Catalog # | A0760-40H2 | | Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 0.25-0.5ug/ml | | Immunohistochemistry (paraffin): 0.5-1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1.2% sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 10K97 | | Host | Mouse | | Concentration | As reported | | Form | Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | N-terminal peptide slightly modified b-cytoplasmic actin, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys (KLH). | | Specificity | Recognizes Beta-Actin. Species Crossreactivity: human, mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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