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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, beta

Anti -Actin, beta

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one bactin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.
Catalog #A0760-41A
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1: 50,00 Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute in 100ul PBS.
PuritySerum.
ImmunogenPurified (>95%) human platelet (nonmuscle) beta-actin protein.
SpecificityRecognizes human Beta-Actin. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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