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You are here:Home » Molecular Biology » Cloning-Primers » Actin beta, Primer Pair, Mouse, Rat (ACTB, Actin, Cytoplasmic 1, Beta-actin)

Actin beta, Primer Pair, Mouse, Rat (ACTB, Actin, Cytoplasmic 1, Beta-actin)

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Specifications

Mouse/rat -actin specific PCR primers for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.
Catalog #A0760-41B
ComponentsPrimer Pair: Lyophilized. Each vial contains 375 pmoles of each primer. Adjust to a final concentration of 7.5 pmoles/ul by resuspending Primer Pair in either 50ul autoclaved deionized water or 0.1X TE buffer (1mM Tris HCl, pH8.0 @ 25°C; 0.1mM EDTA, pH 8.0 @ 25°C).
Positive Control 15 Lyophilized. Each vial contains 150 ng synthetic double-stranded DNA. Resuspend in 30ul autoclaved deionized water or TE buffer. To prevent contaminating samples and reagents, it is strongly recommended that the Positive Control is resuspended in a separate location from where PCR reactions are set up. Use a different pipette than one used for PCR set up. The Positive Control is not intended for quantitative purposes.
GenBank Accession NumberX03672 (mouse) and V01217 (rat)
Product SizescDNA: 302 base pairs (bp)
Genomic DNA: Product predicted and observed at 766 bp. Product observed from pseudogenes in mouse at 302 bp. Treatment of RNA with RNase-free DNase is recommended to remove contaminating genomic DNA.
Positive Control 15: 380 bp
Alternate splice variants: None Reported
Suggested Reaction ConditionsFor each 50ul Polymerase Chain Reaction mixture, 2ul of the primer pair stock solution should be used. Sufficient reagent is supplied for 25 separate amplification reactions of the gene fragment.
Thermocycling ConditionsA typical 50ul PCR would involve a 4 minute denaturation step at 94ºC, followed by 30-35 cycles of 45 seconds at 94ºC, 45 seconds at 55ºC and 45 seconds at 72ºC, with a final 10 minute extension step at 72ºC.
Storage and StabilityThe Primer Pair resuspended in water or TE buffer is stable for up to one year at -20°C in a non-frost free freezer. Aliquot in single use portions. Do not use past the expiration date above. Avoid repeated freeze-thaw cycles.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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