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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, beta

Anti -Actin, beta

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC
Beta actin also know as Cytoplasmic Actin is a 43kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Inhibition of glycolysis with 2-deoxyglucose induced a rapid transformation of -actin-rich pseudopodia and prevented cell motility, therefore a localized glycolytic supply of energy regulates the formation of -actin-rich pseudopodial protrusions and thereby the motility of invasive tumor cells (2). -Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of -Actin is generally used as an internal control for experimental error.
Catalog #A0760-41C
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Immunohistochemistry: 1:50
Immunocytochemistry: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E116
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Beta Actin (Cytoplasmic Actin).
SpecificityRecognizes human Actin, beta. Species crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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