Anti -Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
B IH IC FC IF |
|
| Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle b- and g-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). a-cardiac and a-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, a- and b-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6). | | | Catalog # | A0760-41H | | Applications | Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:300 | | Flow Cytometry: 1:200 | | Western Blot: 1:1000 | | Immunohistochemistry: 1:4000 | | Immunocytochemistry: 1:300 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 10g128 | | Host | Mouse | | Source | Human | | Concentration | Not determined. | | Form | Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol. | | Purity | Ascites | | Immunogen | Synthetic peptide corresponding to amino-terminal residues of human b-actin. | | Specificity | Recognizes endogenous levels of human total beta-actin protein. Species Crossreactivity: Hamster, mouse, rat monkey. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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