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A0760-41N Rabbit Anti-Actin, ID (Actin, cytoplasmic 1, Beta-actin, Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTC1, ACTB/ACTC)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P60709, P68032
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_001092; NP_005150
Shipping Temp
Blue Ice
Storage Temp
-20°C

Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:1000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to a region between corresponding to a portion of amino acids within aa194-224 from the center region of human Actin conjugated to KLH.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human Actin.
References
1. Villebeck,L., Biochemistry 46 (44), 12639-12647 (2007). 2. Avizienyte,E., Exp. Cell Res. 313 (15), 3175-3188 (2007). 3. Bouldin,A.A., Muscle Nerve 35 (2), 254-258 (2007).
USBio References
No references available
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