| Beta actin is one of six different actin isoforms which have been identified. ACTB is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. |
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| Catalog # | A0760-43 |
| Applications | Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:500 |
| Immunohistochemistry: 1:200 |
| Immunocytochemistry: 1:200 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906: IgY, Chicken Pab Rb x Ch |
| I1906-10: IgY, Chicken-HRP Rb x Ch |
| I1906-12: IgY, Chicken-FITC Rb x Ch |
| I1906-14: IgY, Chicken-AP Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide |
| Purity | Purified by affinity chromatography. |
| Immunogen | Recombinant protein corresponding to aa1-375 from human beta actin (4501885, NP001092.1). |
| Specificity | Recognizes human beta Actin, cytoskeletal. |
| Species Crossreactivity | Mouse, rat, rabbit, chicken, bovine and many other species |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
