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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Catalog #	A0760-43A
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 0.1-1.0ug/ml
	Western Blot: 0.1-1.0ug/ml
	Optimal dilutions to be determined by the researcher.
Hybridoma	Sp2/0-AG14 mouse myeloma cells with spleen cells.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	8A103
Host	Mouse
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.01% thimerosal. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 80ul sterile ddH2O.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Synthetic peptide corresponding to DDDIAALVVDNGSG from beta Actin conjugated to KLH
Specificity	Recognizes beta Actin. Species Crossreactivity: mouse, rabbit, chicken, human, hamster, bovine, goat, fish and porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.