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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B

Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle, one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.
Catalog #	A0760-43B
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:5000-1:10,000 using 50-200ng of A0760-44D control peptide/well.
	Western Blot: 1:10500-1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9j104
Host	Mouse
Source	Human
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% merthiolate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 100ul sterile PBS.
Purity	Purified.
Immunogen	Synthetic peptide corresponding to 15aa from human beta Actin (non-muscle) conjugated to KLH. Epitope
Specificity	Recognizes human beta Actin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.