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You are here:Home » Molecular Biology » MB-Actin Related Proteins » Actin, beta Human

Actin, beta Human

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Specifications

Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (gsmooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western Blots.
Catalog #A0760-44F
Biological ActivityNot tested. If the protein is stored and used properly can be used for biological assays.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1ug/ml Used to coat plates
Western Blot: 100-500ng/lane in SDS-PAGE sample buffer (reduced) and load as needed.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~43kD
SourceHuman platelets cells.
PurityPurified (~99%).
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 5mM Tris. pH 8, 0.2mM calcium chloride, 0.2mM ATP, 5% sucrose, 1% dextran. Reconstitute with 100ul sterile ddH2O
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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