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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH

Actin, an ubiquitous protein in eukaryotes, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle beta- and gamma-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. Alpha-cardiac and alpha-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, alpha- and gamma-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells. Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin. The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments. It has been reported that actin is hyperphosphorylated in primary breast tumors. Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle. Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis.
Catalog #	A0760-51
Applications	Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunoprecipitation (Denatured verified) (Use Protein G): 2ug/mg protein lysate
	Western Blot: 0.5ug/ml for 2hrs at RT
	Immunohistochemistry (formalin/paraffin): 1:200 for 30 min
	Requires boiling tissues sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20min.
	Optimal dilutions to be determined by the researcher.
	Positive Control:
	HeLa cells and skeletal muscle.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3F12
Host	Mouse
Source	Chicken
Concentration	~0.1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide before the addition of glycerol to 40%. Also available without BSA and azide. See A0760-51X
Purity	Purified by Protein G affinity chromatography.
Immunogen	Chicken gizzard actin. Cellular Localization: Cytoplasmic
Specificity	Recognizes all six known vertebrate isoforms of actin (MW~42kD) and two cytoplasmic actins (beta, gamma) which are highly homologous to one another but which differ from the muscle actins at about 25aa. A0760-51 is highly recommended for monitoring total protein load on Western blots. Species Crossreactivity: Human, bovine, mouse, canine, porcine, rabbit, rat, chicken, Dictyostelium discoideum, Physarum polycephalum.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.