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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, pan

Anti -Actin, pan

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Catalog #A0760-51C
Cellular Localization Cytoplasmic
Positive Control HeLa cells, Skeletal muscle
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 42kD band is seen.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No8J176
HostMouse
SourceChicken
Concentration~0.2mg/ml
Form10mM PBS, pH7.4, 0.2%BSA
PurityPurified by Protein G affinity chromatography.
ImmunogenChicken gizzard actin.
SpecificityThis antibody reacts with all six known vertebrate isoforms of actin (MW 42kDa). It also reacts with two cytoplasmic actins (beta, lambda) which are highly homologous to one another but which differ from the muscle actins at about 25 amino acid residues. This antibody is highly recommended for monitoring total protein load on Western blots. Species Crossreactivity: human, chicken, bovine, canine, mouse, porcine, rabbit, Rat, Dictyostelium discoideum and Physarum polycephalum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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