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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH IF

Catalog #	A0760-51C
Cellular Localization	Cytoplasmic
Positive Control	HeLa cells, Skeletal muscle
Applications	Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 42kD band is seen.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	8J176
Host	Mouse
Source	Chicken
Concentration	~0.2mg/ml
Form	10mM PBS, pH7.4, 0.2%BSA
Purity	Purified by Protein G affinity chromatography.
Immunogen	Chicken gizzard actin.
Specificity	This antibody reacts with all six known vertebrate isoforms of actin (MW 42kDa). It also reacts with two cytoplasmic actins (beta, lambda) which are highly homologous to one another but which differ from the muscle actins at about 25 amino acid residues. This antibody is highly recommended for monitoring total protein load on Western blots. Species Crossreactivity: human, chicken, bovine, canine, mouse, porcine, rabbit, Rat, Dictyostelium discoideum and Physarum polycephalum.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.